MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression

MiR-490-5p Restrains Progression of Gastric cancer through DTL Repression

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Gastric cancer (GC) accounts for a main cause of cancer-related deaths.This study sought for molecular mechanism of miR-490-5p/DTL axis in affecting GC progression, thus bringing new hope for treatment of GC.Expression data of differentially expressed miRNAs and mRNAs in GC tissue from TCGA database were analyzed.MiR-490-5p and DTL Bottle Bag mRNA expression levels in GC were evaluated with qRT-PCR.Cell viability was confirmed with CCK-8 method.

Cell cycle distribution and apoptosis were analyzed with flow cytometry.Cell migratory and invasive potential was proved with Transwell assay.The targeted relationship between DTL and miR-490-5p was analyzed with dual-luciferase assay.The ORG BANANA CHIPS results indicated a decreased miR-490-5p level in GC cells.MiR-490-5p upregulation hampered proliferation, migration, invasion and promote cell apoptosis.

DTL was the target of and inversely associated with miR-490-5p, and it could remarkably induce the carcinogenesis of GC.MiR-490-5p mediated GC cell progression by DTL repression.In conclusion, miR-490-5p and DTL may be valuable in diagnosis and treatment for GC.